PMC

Maximum likelihood phylogenetic tree of the E2 family, including charge conservation models at the E2^HTH.Shannon entropy plot of residue conservation across the E2 family. The majority of residues in the E2^HTH (pink stars) have increased Shannon entropy compared to the E2 mean represented by the horizontal line.Weblogos of E2^HTH residue conservation for each E2 family member. UBE2S, UBE2C, UBE2D, and UBE2R are repeated from Fig  for comparison.

A, charge movement currents recorded in response to depolarizing pulses to −10 and +20 mV of increasing duration (2 ms increments) to evaluate charge movement conservation. B, Q_ON or Q_OFFvs. pulse duration for the complete set of pulses of corresponding traces illustrated in A. The normalized charge-pulse duration relationships for both Q_ON and Q_OFF are fitted to single exponential functions (continuous lines). The calculated Q_ON time constants were 12.7 ms for pulses to −10 mV, and 2.3 ms for pulses to +20 mV.

Charge conservation in Kv4.2_CTX and Kv4.2_CTX:DPPX-S channels. (A) Q_ON-V and Q_OFF-V relationships from Kv4.2_CTX channels. (B) Q_ON-V and Q_OFF-V relationships from Kv4.2_CTX:DPPX-S channels. (C) Q_ON and Q_OFF values from Kv4.2_CTX channels are strongly correlated with a slope of 0.97 (r = 0.98). (D) Q_ON and Q_OFF values from Kv4.2_CTX:DPPX-S channels are strongly correlated with a slope of 1.03 (r = 0.99).

(A) Normalized conservation scores (labelled rate of evolution) per site overlaid on the functional selection-expected charge plot. These conservation scores were obtained from Rate4site [] with H1N1 sequences from the fludb database of the IRD []. A higher score indicates lower conservation and faster evolution. (B) Empirical cumulative distributions of conservation scores for each of the three branches. (C) Distances to the RBS overlaid on the functional selection-expected charge plot. These distances were calculated using PyMOL [] (see ) (D) Empirical cumulative distributions of the distance to the RBS for each of the three branches. (E) Relative solvent accessibility overlaid on the functional selection-expected charge plot. These scores were computed using dmstools2 [] (see ) (F) Empirical cumulative distributions of relative solvent accessibility for each of the three branches.